Journal: Purinergic Signalling

Article Title: Abnormal purinergic signaling contributes to development of renal cysts in autosomal dominant polycystic kidney disease

doi: 10.1007/s11302-025-10127-1

Figure Lengend Snippet: Effect of P2X7 receptors stimulation on cyst growth in 3D cell culture. A Representative image of cysts formed by mpkCCD cl4 cells in the presence of 10 µM forskolin. B Treatment with α,βMe-ATP (P2X agonist) increases the size of cysts grown from mpkCCD cl4 cells. C Western blot analysis of pannexin-1 abundance in untreated (no forskolin in Matrigel), and vehicle or α,βMe-ATP treated groups in the presence of forskolin. Summary graphs on the right panels denote that stimulation with α,βMe-ATP increases pannexin-1 abundance. N = 4 wells in each group, n = 50 cysts in each well. D Representative images of MDCK cells grown in Matrigel in the absence ( left ) or presence ( right ) of 10 µM forskolin. Treatment of MDCK cysts with 100 µM α,βMe-ATP or 50 µM BzATP aggravates cystogenesis ( n = 128–232 cysts/well) ( E ) and increases the abundance of pannexin-1 ( F ). * p < 0.05; ** p < 0.01; *** p < 0.001 vs forskolin + vehicle; Shapiro–Wilk (passed) Dunnett test

Article Snippet: Key experiments were reproduced in canine renal epithelial MDCK cells (ATCC CCL-34).

Techniques: Cell Culture, Western Blot

Journal: Purinergic Signalling

Article Title: Abnormal purinergic signaling contributes to development of renal cysts in autosomal dominant polycystic kidney disease

doi: 10.1007/s11302-025-10127-1

Figure Lengend Snippet: Effect of SIL14 treatment on cyst growth in 3D cell culture. MpkCCD cl4 or MDCK cells were grown in Matrigel in the presence of 10 µM forskolin. A Confocal microscopy shows the distribution of pannexin-1 and phalloidin in the plasma membrane of the mpkCCD cl4 cyst epithelium. B , C Treatment with SIL14 reduces mpkCCD cl4 cyst size. n = 4 wells, n = 112–150 cysts/well. ** p < 0.01 vs vehicle, Shapiro–Wilk (passed), Dunnett test. D Representative image of cysts formed by MDCK cells in the presence of 30 µM SIL14. ( E ) Summary graph demonstrates that SIL14 reduces cyst size in comparison to the vehicle-treated group. N = 4 wells, n = 90–150 cysts/well. * p < 0.05 unpaired t -test

Article Snippet: Key experiments were reproduced in canine renal epithelial MDCK cells (ATCC CCL-34).

Techniques: Cell Culture, Confocal Microscopy, Clinical Proteomics, Membrane, Comparison